Genetic Engineering In Plants Essay About Myself

1. Southgate EM, Davey MR, Power JB, Merchant R. Factors affecting the genetic engineering of plants by microprojectile bombardment. Biotechnol Adv. 1995;13:631–57.[PubMed]

2. Sticklen M. Plant genetic engineering to improve biomass characteristics for biofuels. Curr Opin Biotechnol. 2005;17:315–9.[PubMed]

3. Conrad U. Polymers from plants to develop biodegradable plastics. Trends Plant Sci. 2005;10:511–2.[PubMed]

4. Ma JKC, Drake PMW, Christou P. The production of recombinant pharmaceutical proteins in plants. Nature. 2003;4:794–805.[PubMed]

5. ISAAA Briefs No. 37. Ithaca, NY: ISAAA; 2007. Executive summary of Global Status of Commercialised Biotech/GM crops: 2007.

6. Brandt P. Overview of the current status of genetically modified plants in Europe as compared to the USA. J Plant Physiol. 2003;160:735–40.[PubMed]

7. Gay PB, Gillespie SH. Antibiotic resistance markers in genetically modified plants; a risk to human health. Lancet Infect Dis. 2005;5:637–46.[PubMed]

8. Bennett PM, Livesey CT, Nathwani D, Reeves DS, Saunders JR, Wise R. An assessment of the risks associated with the use of antibiotic resistance genes in genetically modified plants: report of the Working Party of the British Society for Antimicrobial Chemotherapy. J Antimicrob Chemother. 2004;53:418–31.[PubMed]

9. Goldstein DA, Tinland B, Gilbertson LA, et al. Human safety and genetically modified plants: a review of antibiotic resistance markers and future transformation selection technologies. J App Microbiol. 2005;99:7–23.[PubMed]

10. Hare PD, Chua NH. Excision of selectable marker genes from GM plants. Nat Biotech. 2002;20:575–80.[PubMed]

11. Wu HX, Sparks CA, Jones HD. Characterisation of T-DNA loci and vector backbone sequences in GM wheat produced by Agrobacterium-mediated transformation. Mol Breed. 2006;18:195–208.

12. Fu XD, Duc LT, Fontana S, et al. Linear transgene constructs lacking vector backbone sequences generate low-copy-number GM plants with simple integration patterns. Transgenic Res. 2000;9:11–9.[PubMed]

13. Latham JR, Wilson AK, Steinbrecher RA. The mutational consequences of plant transformation. J Biomed Biotech. 2006:1–7. Article ID 25376.

14. Pinstrup-Anderson P, Pandra-Lorch R, Rosegrant MW. World food prospects: critical issues for the early twenty-first century. 1999 Food policy report. Washington DC: International Food Policy Research Institute; 1999.

15. Food and Agriculture Organisation. The State of Food Insecurity in the World. Rome: FAO; 2001.

16. Smith LC, El Obeid AE, Jensen HH. The geography and causes of food insecurity in developing countries. Agriculture Economics. 2000;22:199–215.

17. World Bank. World Development report 2000/2001. Attacking poverty. Washington DC: World Bank; 2000.

18. Christou P, Twyman RM. The potential of genetically enhanced plants to address food insecurity. Nut Research Rev. 2004;17:23–42.[PubMed]

19. Byrnes BH, Bumb BL. Population growth, food production and nutrient requirements. J Crop Prod. 1998;1:1–27.

20. Byerlee D, Helsey P, Pingali PL. Realising yield gains for food staples in developing countries in the early 21st century: prospects and challenges. In: Chang BM, Colombo M, Soronolo M, editors. Food Needs of the Developing World in the 21st Century. Vatican City: Political Academy of Sciences; 2000. pp. 207–50.

21. Rosegrant MW, Paisner MS, Mejer S, Witcover J. 2020 Global Food Outlook Trends, Alternatives and Choices. A 2020 Vision for Food Agriculture and the Environment Initiative. Washington DC: IFPRI; 2001.

22. World Health Organisation. Combating vitamin A deficiency. Geneva: WHO; 2001. www.who.int/nut/vad.htm.

23. Ye XD, Al-Babili S, Kloti A, et al. Engineering the provitamin A (β-carotene) biosynthetic pathway into (carotenoid-free) rice endosperm. Science. 2000;287:303–5.[PubMed]

24. Paine JA, Shipton CA, Chaggar S, et al. Improving the nutritional content of Golden Rice through increased provitamin A content. Nat Biotechnol. 2005;23:482–7.[PubMed]

25. Potrykus I. Golden Rice and beyond. Plant Physiol. 2001;125:1157–61.[PMC free article][PubMed]

26. Anderson PK, Cunningham AA, Patel NG, Morales FJ, Epstein PR, Daszak P. Emerging infectious diseases of plants: pathogen, pollution, climate change and agrotechnology drivers. Trends Eco Evol. 2004;19:535–44.[PubMed]

27. Gurr SJ, Rushton PJ. Engineering plants with increased disease resistance: what are we going to express? Trends Biotech. 2005;23:275–82.[PubMed]

28. Brookes G, Barfoot P. ISAAA briefs. ISAAA; 2006. http://www.isaaa.org.

29. Peschen D, Li HP, Fischer R, Kreuzaler F, Liao YC. Fusion protein comprising a Fusarium- specific antibody linked to antifungal peptides protect plants against a fungal pathogen. Nat Biotech. 2004;22:732–8.[PubMed]

30. Tai TH, Dahlbeck D, Clark ET, et al. Expression of Bs2 pepper gene confers resistance to bacterial spot disease in tomatoes. Proc Natl Acad Sci USA. 1999;96:14153–8.[PMC free article][PubMed]

31. Vinocur B, Altman A. Recent advances in engineering plant tolerance to abiotic stress: achievements and limitations. Curr Op Biotech. 2005;16:123–32.[PubMed]

32. Ashraf M. Breeding for salinity tolerance in plants. Crit Rev Plant Sci. 1994;13:17–42.

33. Shou H, Bordallo P, Fan FB, et al. Expression of an active tobacco mitogen activated protein kinase kinase kinase enhances freezing tolerance in GM maize. Proc Natl Acad Sci USA. 2004;101:3298–303.[PMC free article][PubMed]

34. Guidance document of the genetically modified organisms for the risk assessment of genetically modified plants and derived food and feed. EFSA J. 2004;99:1–94.

35. McKeon TA. Genetically modified crops for industrial products and processes and their effects on human health. Trends Food Sci Tech. 2003;14:229–41.

36. Ewen SWB, Pusztai A. Effects of diets containing genetically modified potatoes expressing Galanthus Nivalis lectin on rat small intestine. Lancet. 1999;354:1353–4.[PubMed]

37. Burke DM. GM Food and Crops: what went wrong in the UK? EMBO Reports. 2004;5:432–6.[PMC free article][PubMed]

38. FAO/WHO. Evaluation of Allergenicity of Genetically Modified Foods, Report of a joint FAO/WHO Expert Consultation on Allergenicity of Foods derived from Biotechnology. Geneva: FAO/WHO; 2001.

39. Doerfler W, Schubbert R, Fremde DNA im Sciugersystem. Deutsches Arzteblatt. 1997;94:51–2.

40. Malarkey T. Human Health concerns with GM crops. Mut Res. 2003;544:217–22.[PubMed]

41. WHO/FAO. Strategies for assessing the safety of foods produced by biotechnology. Report of the Joint WHO/FAO Consultation. Geneva: FAO/WHO; 1991.

42. FDA Statement of policy, foods derived from new plant varieties. Fed Reg. 1992;57

43. OECD. Food Safety Evaluation. Paris: OECD Documents; 1996.

44. Prescott VE, Campbell PM, Moore A, et al. Transgenic expression of bean α-amylase inhibitor in peas results in altered structure and immunogenicity. J Agric Food Chem. 2005;53:9023–30.[PubMed]

45. Nordlee JA, Taylor SL, Townsend JA, Thomas LA, Bush RK. Identification of a brazil-nut allergen in GM soybeans. NEJM. 1996;334:688–92.[PubMed]

46. Herman E. Soybean allergenicity and suppression of the immunodominant allergen. Crop Sci. 2005;45:462–7.

47. Sijmons PC, Dekker BM, Schranmeijer B, Verwoerd TC, van den Elzen PJ, Hoekema A. Production of correctly processed human serum albumin in GM plants. Biotechnology. 1990;8:217–21.[PubMed]

48. Twyman RM, Schillberg S, Fischer R. Transgenic plants in the biopharmaceutical market. Expert Opin Emerg Drugs. 2005;10:185–218.[PubMed]

49. Kapusta J, Modelska A, Figlerowicz M, et al. A plant-derived edible vaccine against hepatitis B virus. FASED J. 1999;13:1796–9.[PubMed]

50. Tacket CO, Mason HS, Losonsky G, Clements JD, Levine MM, Arntzen CJ. Immunogenicity in humans of a recombinant bacterial antigen delivered in GM potato. Nature Med. 1998;4:607–9.[PubMed]

51. Tacket CO, Mason HS, Losonsky G, Estes MK, Levine MM, Arntzen CJ. Human responses to a novel Norwalk virus vaccine delivered in GM potatoes. J Infect Dis. 2000;182:302–5.[PubMed]

52. http://www.i-sis.org.uk/gmSaffloweHumanPro-Insulin.php

53. Hiatt A, Cafferkey R, Bowdish K. Production of antibodies in GM plants. Nature. 1998;342:76–8.[PubMed]

54. During K, Hippe S, Kreuzaler F, Schell J. Synthesis and self assembly of a functional monoclonal antibody in GM Nicotiana tabacum. Plant Mol Biol. 1990;15:281–93.[PubMed]

55. Ma JK-C, Hiatt A, Hein M, et al. Generation and assembly of secretory antibodies in plants. Science. 1995;268:716–9.[PubMed]

56. Francisco JA, Gawlak SL, Miller M, et al. Expression and characterisation of bryodin 1 and a bryodin-based single-chain immunotoxin from tobacco cell culture. Bioconjug Chem. 1997;8:708–13.[PubMed]

57. Mayfield SP, Franklin SE, Lerner RA. Expression and assembly of a fully active antibody in algae. Proc Natl Acad Sci USA. 2003;100:438–42.[PMC free article][PubMed]

58. EMBO Reports. Molecular farming for new drugs and vaccines. EMBO Reports. 2005;6:593–9.[PMC free article][PubMed]

59. Fox JL. Puzzling industry response to Prodigene fiasco. Nat Biotech. 2003;21:3–4.[PubMed]

60. Quist D, Chapela JH. Transgenic DNA introgressed into traditional maize landraces in Oaxaca, Mexico. Nature. 2001;414:541–3.[PubMed]

61. Kaplinsky N, Braun D, Lisch D, Hay A, Hake S, Freeling M. Biodiversity (communications arising): maize transgene results in Mexico are artefacts. 2002. p. 601. [PubMed]

62. Metz M, Fulterer J. Biodiversity (communications arising): suspect evidence of GM contamination. Nature. 2002;416:600–1.[PubMed]

63. Ortiz-Garcia S, Ezcurra E, Schoel B, Acevedo F, Soberon J, Snow AA. Absence of detectable transgenes in local landacres of maize in Oaxaca, Mexico (2003–2004) Proc Natl Acad Sci USA. 2005;102:12338–43.[PMC free article][PubMed]

64. Reichman JR, Watrud LS, Lee EH, et al. Establishment of GM herbicide-resistance creeping bentgrass (Agrostis Stolonifera L.) in nonagronomic habitats. Mol Ecol. 2006;15:4243–55.[PubMed]

65. Losey JE, Rayor LS, Carter ME. Transgenic pollen harms monarch larvae. Nature. 1999;399:214.[PubMed]

66. Sears MK, Hellmich RL, Stanley-Horn DE, et al. Impact of Bt corn pollen on monarch butterfly populations: a risk assessment. Proc Natl Acad Sci USA. 2001;98:11937–42.[PMC free article][PubMed]

67. Pleasants JM, Hellmich RL, Dively GP, et al. Corn pollen deposition on milkweeds in and near cornfields. Proc Natl Acad Sci USA. 2001;98:11919–24.[PMC free article][PubMed]

68. Stanley-Horn DE. Assessing the impact of Cry1Ab expressing corn pollen on monarch larvae in field studies. Proc Natl Acad Sci USA. 2001;119:31–36.[PMC free article][PubMed]

69. www.defra.gov.uk/environment/gm/fse

70. Sasaki Y, Hayakawa T, Inoue C, Miyazaki A, Silver S, Kusano T. Generation of mercury hyper-accumulating plants through GM expression of the bacterial mercury membrane transport protein MerC. Transgenic Res. 2006;15:615–25.[PubMed]

71. Banuelos G, Leduc DL, Pilon-Smits EAH, Terry N. Transgenic Indian mustard overexpressing selenocysteine lyase or seloncystiene methyltransferase exhibit enhanced potential for selenium phytoremediation under field conditions. Environ Sci Tech. 2007;41:599–605.[PubMed]

72. Mascia PN, Flovell RB. Safe and acceptable strategies for producing foreign materials in plants. Curr Opin Plant Biol. 2004;7:189–95.[PubMed]

73. Daniell H. GM Crops: public perception and scientific solutions. Trends Plant Sci. 1999;4:467–9.[PubMed]

74. Five Year Freeze Campaign. Why a Five Year Freeze?http://www.fiveyearfreeze.org/indexb.htm.

75. Zhang HX, Blumwald E. Transgenic salt-tolerant tomato plants accumulate salt in foliage but not in fruit. Nat Biotechnol. 2001;19:765–8.[PubMed]

76. Taverne D. The new fundamentalism. Nat Biotechnol. 2005;23:415–6.[PubMed]

77. Von Emden HF, Gray A, editors. GMOs – Ecological Dimensions. Wellsbourne, UK: Association of Applied Biologists;

78. Bradford KJ, Deynze AV, Gutterson N, Parrott W, Strauss SH. Regulating GM crops sensibly: lessons from plant breeding biotechnology and genomics. Nat Biotechnol. 2005;23:439–44.[PubMed]

79. Kenward KD, Altshuler M, Davies PL. Accumulation of type 1 fish antifreeze protein in GM tobacco is cold specific. Plant Mol Biol. 1993;23:377–85.[PubMed]

80. Consumer Watch. GM Food and Farming: What are Consumer's latest views? Watford, UK: IGD; 2003.

81. Poortinga W, Pidgeon NF. Public perceptions of Genetically Modified Food and Crops, and the GM nation? Norfolk, UK: Centre for Environmental Risk, Norwich; 2004. (Understanding risk, working paper 04–01.)

82. Gorden G. GM Crops opposition may have been ‘over-estimated’ Scotsman. 2004 Feb 19;

83. Better dead than GM feed? Economist. 2002 Mar 13;31

Genetic modification caused by human activity has been occurring since around 12,000 BC, when humans first began to domesticate organisms. Genetic engineering as the direct transfer of DNA from one organism to another was first accomplished by Herbert Boyer and Stanley Cohen in 1972. The first genetically modified animal was a mouse created in 1974 by Rudolf Jaenisch. In 1983 an antibiotic resistant gene was inserted into tobacco, leading to the first genetically engineered plant. Advances followed that allowed scientists to manipulate and add genes to a variety of different organisms and induce a range of different effects.

In 1976 the technology was commercialized, with the advent of genetically modified bacteria that produced somatostatin, followed by insulin in 1978. Plants were first commercialized with virus resistant tobacco released in China in 1992. The first genetically modified food was the Flavr Savr tomato marketed in 1994. By 2010, 29 countries had planted commercialized biotech crops. In 2000 a paper published in Science introduced golden rice, the first food developed with increased nutrient value.

Agriculture[edit]

Main article: History of agriculture

Genetic engineering is the direct manipulation of an organism's genome using certain biotechnology techniques that have only existed since the 1970s.[2] Human directed genetic manipulation was occurring much earlier, beginning with the domestication of plants and animals through artificial selection. The dog is believed to be the first animal domesticated, possibly arising from a common ancestor of the grey wolf,[1] with archeological evidence dating to about 12,000 BC.[3] Other carnivores domesticated in prehistoric times include the cat, which cohabited with human 9,500 years ago.[4] Archeological evidence suggests sheep, cattle, pigs and goats were domesticated between 9 000 BC and 8 000 BC in the Fertile Crescent.[5]

The first evidence of plant domestication comes from emmer and einkorn wheat found in pre-Pottery Neolithic A villages in Southwest Asia dated about 10,500 to 10,100 BC. The Fertile Crescent of Western Asia, Egypt, and India were sites of the earliest planned sowing and harvesting of plants that had previously been gathered in the wild. Independent development of agriculture occurred in northern and southern China, Africa's Sahel, New Guinea and several regions of the Americas.[7] The eight Neolithic founder crops (emmer wheat, einkorn wheat, barley, peas, lentils, bitter vetch, chick peas and flax) had all appeared by about 7000 BC.[8]Horticulture first appears in the Levant during the Chalcolithic period about 6 800 to 6,300 BC. Due to the soft tissues, archeological evidence for early vegetables is scarce. The earliest vegetable remains have been found in Egyptian caves that date back to the 2nd millennium BC.

Selective breeding of domesticated plants was once the main way early farmers shaped organisms to suit their needs. Charles Darwin described three types of selection: methodical selection, wherein humans deliberately select for particular characteristics; unconscious selection, wherein a characteristic is selected simply because it is desirable; and natural selection, wherein a trait that helps an organism survive better is passed on.[11]:25 Early breeding relied on unconscious and natural selection. The introduction of methodical selection is unknown.[11]:25 Common characteristics that were bred into domesticated plants include grains that did not shatter to allow easier harvesting, uniform ripening, shorter lifespans that translate to faster growing, loss of toxic compounds, and productivity.[11]:27–30 Some plants, like the Banana, were able to be propagated by vegetative cloning. Offspring often did not contain seeds, and therefore sterile. However, these offspring were usually juicier and larger. Propagation through cloning allows these mutant varieties to be cultivated despite their lack of seeds.[11]:31

Hybridization was another way that rapid changes in plant's makeup were introduced. It often increased vigor in plants, and combined desirable traits together. Hybridization most likely first occurred when humans first grew similar, yet slightly different plants in close proximity.[11]:32Triticum aestivum, wheat used in baking bread, is an allopolyploid. Its creation is the result of two separate hybridization events.[12]

Grafting can transfer chloroplasts (specialised DNA in plants that can conduct photosynthesis), mitichondrial DNA and the entire cell nucleus containing the genome to potentially make a new species making grafting a form of natural genetic engineering.[13]

X-rays were first used to deliberately mutate plants in 1927. Between 1927 and 2007, more than 2,540 genetically mutated plant varieties had been produced using x-rays.[14]

Genetics[edit]

Main article: History of genetics

Various genetic discoveries have been essential in the development of genetic engineering. Genetic inheritance was first discovered by Gregor Mendel in 1865 following experiments crossing peas. Although largely ignored for 34 years he provided the first evidence of hereditary segregation and independent assortment.[15] In 1889 Hugo de Vries came up with the name "(pan)gene" after postulating that particles are responsible for inheritance of characteristics[16] and the term "genetics" was coined by William Bateson in 1905.[17] In 1928 Frederick Griffithproved the existence of a "transforming principle" involved in inheritance, which Avery, MacLeod and McCarty later (1944) identified as DNA. Edward Lawrie Tatum and George Wells Beadle developed the central dogma that genes code for proteins in 1941. The double helix structure of DNA was identified by James Watson and Francis Crick in 1953.

As well as discovering how DNA works, tools had to be developed that allowed it to be manipulated. In 1970 Hamilton Smiths lab discovered restriction enzymes that allowed DNA to be cut at specific places and separated out on an electrophoresis gel. This enabled scientists to isolate genes from an organism's genome.[18]DNA ligases, that join broken DNA together, had been discovered earlier in 1967[19] and by combining the two enzymes it was possible to "cut and paste" DNA sequences to create recombinant DNA. Plasmids, discovered in 1952,[20] became important tools for transferring information between cells and replicating DNA sequences. Frederick Sanger developed a method for sequencing DNA in 1977, greatly increasing the genetic information available to researchers. Polymerase chain reaction (PCR), developed by Kary Mullis in 1983, allowed small sections of DNA to be amplified and aided identification and isolation of genetic material.

As well as manipulating the DNA, techniques had to be developed for its insertion (known as transformation) into an organism's genome. Griffiths experiment had already shown that some bacteria had the ability to naturally take up and express foreign DNA. Artificial competence was induced in Escherichia coli in 1970 when Morton Mandel and Akiko Higa showed that it could take up bacteriophage λ after treatment with calcium chloride solution (CaCl2).[21] Two years later, Stanley Cohen showed that CaCl2 treatment was also effective for uptake of plasmid DNA.[22] Transformation using electroporation was developed in the late 1980s, increasing the efficiency and bacterial range.[23] In 1907 a bacterium that caused plant tumors, Agrobacterium tumefaciens, was discovered and in the early 1970s the tumor inducing agent was found to be a DNA plasmid called the Ti plasmid.[24] By removing the genes in the plasmid that caused the tumor and adding in novel genes researchers were able to infect plants with A. tumefaciens and let the bacteria insert their chosen DNA into the genomes of the plants.[25]

Early genetically modified organisms[edit]

In 1972 Paul Berg used restriction enzymes and DNA ligases to create the first recombinant DNA molecules. He combined DNA from the monkey virus SV40 with that of the lambda virus.[26] Herbert Boyer and Stanley Norman Cohen took Berg's work a step further and introduced recombinant DNA into a bacterial cell. Cohen was researching plasmids, while Boyers work involved restriction enzymes. They recognised the complementary nature of their work and teamed up in 1972. Together they found a restriction enzyme that cut the pSC101 plasmid at a single point and were able to insert and ligate a gene that conferred resistance to the kanamycin antibiotic into the gap. Cohen had previously devised a method where bacteria could be induced to take up a plasmid and using this they were able to create a bacteria that survived in the presence of the kanamycin. This represented the first genetically modified organism. They repeated experiments showing that other genes could be expressed in bacteria, including one from the toad Xenopus laevis, the first cross kingdom transformation.[27][28][29]

In 1974 Rudolf Jaenisch created a transgenic mouse by introducing foreign DNA into its embryo, making it the world’s first transgenic animal.[30][31] Jaenisch was studying mammalian cells infected with simian virus 40 (SV40) when he happened to read a paper from Beatrice Mintz describing the generation of chimera mice. He took his SV40 samples to Mintz's lab and injected them into early mouse embryos expecting tumours to develop. The mice appeared normal, but after using radioactive probes he discovered that the virus had integrated itself into the mice genome.[32] However the mice did not pass the transgene to their offspring. In 1981 the laboratories of Frank Ruddle, Frank Constantini and Elizabeth Lacy injected purified DNA into a single-cell mouse embryo and showed transmission of the genetic material to subsequent generations.[33][34]

The first genetically engineered plant was tobacco, reported in 1983.[35] It was developed by Michael W. Bevan, Richard B. Flavell and Mary-Dell Chilton by creating a chimeric gene that joined an antibiotic resistant gene to the T1 plasmid from Agrobacterium. The tobacco was infected with Agrobacterium transformed with this plasmid resulting in the chimeric gene being inserted into the plant. Through tissue culture techniques a single tobacco cell was selected that contained the gene and a new plant grown from it.[36]

Regulation[edit]

Main article: Regulation of genetic engineering

The development of genetic engineering technology led to concerns in the scientific community about potential risks. The development of a regulatory framework concerning genetic engineering began in 1975, at Asilomar, California. The Asilomar meeting recommended a set of guidelines regarding the cautious use of recombinant technology and any products resulting from that technology.[37] The Asilomar recommendations were voluntary, but in 1976 the US National Institute of Health (NIH) formed a recombinant DNA advisory committee.[38] This was followed by other regulatory offices (the United States Department of Agriculture (USDA), Environmental Protection Agency (EPA) and Food and Drug Administration (FDA), effectively making all recombinant DNA research tightly regulated in the USA.[39]

In 1982 the Organization for Economic Co-operation and Development (OECD) released a report into the potential hazards of releasing genetically modified organisms into the environment as the first transgenic plants were being developed.[40] As the technology improved and genetically organisms moved from model organisms to potential commercial products the USA established a committee at the Office of Science and Technology (OSTP) to develop mechanisms to regulate the developing technology.[39] In 1986 the OSTP assigned regulatory approval of genetically modified plants in the US to the USDA, FDA and EPA.[41] In the late 1980s and early 1990s, guidance on assessing the safety of genetically engineered plants and food emerged from organizations including the FAO and WHO.[42][43][44][45]

The European Union first introduced laws requiring GMO's to be labelled in 1997.[46] In 2013 Connecticut became the first state to enact a labeling law in the USA, although it would not take effect until other states followed suit.[47]

Research and Medicine[edit]

The ability to insert, alter or remove genes in model organisms allowed scientists to study the genetic elements of human diseases.[48]Genetically modified mice were created in 1984 that carried cloned oncogenes that predisposed them to developing cancer.[49] The technology has also been used to generate mice with genes knocked out. The first recorded knockout mouse was created by Mario R. Capecchi, Martin Evans and Oliver Smithies in 1989. In 1992 oncomice with tumor suppressor genes knocked out were generated.[49] Creating Knockout rats is much harder and only became possible in 2003.[50][51]

After the discovery of microRNA in 1993,[52]RNA interference (RNAi) has been used to silence an organism's genes.[53] By modifying an organism to express microRNA targeted to its endogenous genes, researchers have been able to knockout or partially reduce gene function in a range of species. The ability to partially reduce gene function has allowed the study of genes that are lethal when completely knocked out. Other advantages of using RNAi include the availability of inducible and tissue specific knockout.[54] In 2007 microRNA targeted to insect and nematode genes was expressed in plants, leading to suppression when they fed on the transgenic plant, potentially creating a new way to control pests.[55] Targeting endogenous microRNA expression has allowed further fine tuning of gene expression, supplementing the more traditional gene knock out approach.[56]

Genetic engineering has been used to produce proteins derived from humans and other sources in organisms that normally cannot synthesize these proteins. Human insulin-synthesising bacteria were developed in 1979 and were first used as a treatment in 1982.[57] In 1988 the first human antibodies were produced in plants.[58] In 2000 Vitamin A-enriched golden rice, was the first food with increased nutrient value.[59]

Further advances[edit]

As not all plant cells were susceptible to infection by A. tumefaciens other methods were developed, including electroporation, micro-injection[60] and particle bombardment with a gene gun (invented in 1987).[61][62] In the 1980s techniques were developed to introduce isolated chloroplasts back into a plant cell that had its cell wall removed. With the introduction of the gene gun in 1987 it became possible to integrate foreign genes into a chloroplast.[63]

Genetic transformation has become very efficient in some model organisms. In 2008 genetically modified seeds were produced in Arabidopsis thaliana by simply dipping the flowers in an Agrobacterium solution.[64] The range of plants that can be transformed has increased as tissue culture techniques have been developed for different species.

The first transgenic livestock were produced in 1985,[65] by micro-injecting foreign DNA into rabbit, sheep and pig eggs.[66] The first animal to synthesise transgenic proteins in their milk were mice,[67] engineered to produce human tissue plasminogen activator.[68] This technology was applied to sheep, pigs, cows and other livestock.[67]

In 2010 scientists at the J. Craig Venter Institute announced that they had created the first synthetic bacterial genome. The researchers added the new genome to bacterial cells and selected for cells that contained the new genome. To do this the cells undergoes a process called resolution, where during bacterial cell division one new cell receives the original DNA genome of the bacteria, whilst the other receives the new synthetic genome. When this cell replicates it uses the synthetic genome as its template. The resulting bacterium the researchers developed, named Synthia, was the world's first synthetic life form.[69][70]

In 2014 a bacteria was developed that replicated a plasmid containing an unnatural base pair. This required altering the bacterium so it could import the unnatural nucleotides and then efficiently replicate them. The plasmid retained the unnatural base pairs when it doubled an estimated 99.4% of the time.[71] This is the first organism engineered to use an expanded genetic alphabet.[72]

In 2015 CRISPR and TALENs was used to modify plant genomes. Chinese labs used it to create a fungus-resistant wheat and boost rice yields, while a U.K. group used it to tweak a barley gene that could help produce drought-resistant varieties. When used to precisely remove material from DNA without adding genes from other species, the result is not subject the lengthy and expensive regulatory process associated with GMOs. While CRISPR may use foreign DNA to aid the editing process, the second generation of edited plants contain none of that DNA. Researchers celebrated the acceleration because it may allow them to "keep up" with rapidly evolving pathogens. The U.S. Department of Agriculture stated that some examples of gene-edited corn, potatoes and soybeans are not subject to existing regulations. As of 2016 other review bodies had yet to make statements.[73]

Commercialisation[edit]

In 1976 Genentech, the first genetic engineering company was founded by Herbert Boyer and Robert Swanson and a year later the company produced a human protein (somatostatin) in E.coli. Genentech announced the production of genetically engineered human insulin in 1978.[74] In 1980 the U.S. Supreme Court in the Diamond v. Chakrabarty case ruled that genetically altered life could be patented.[75] The insulin produced by bacteria, branded humulin, was approved for release by the Food and Drug Administration in 1982.[76]

In 1983 a biotech company, Advanced Genetic Sciences (AGS) applied for U.S. government authorization to perform field tests with the ice-minus strain of P. syringae to protect crops from frost, but environmental groups and protestors delayed the field tests for four years with legal challenges.[77] In 1987 the ice-minus strain of P. syringae became the first genetically modified organism (GMO) to be released into the environment[78] when a strawberry field and a potato field in California were sprayed with it.[79] Both test fields were attacked by activist groups the night before the tests occurred: "The world's first trial site attracted the world's first field trasher".[78]

The first genetically modified crop plant was produced in 1982, an antibiotic-resistant tobacco plant.[80] The first field trials of genetically engineered plants occurred in France and the USA in 1986, tobacco plants were engineered to be resistant to herbicides.[81] In 1987 Plant Genetic Systems, founded by Marc Van Montagu and Jeff Schell, was the first company to genetically engineer insect-resistant plants by incorporating genes that produced insecticidal proteins from Bacillus thuringiensis (Bt) into tobacco.[82]

Genetically modified microbial enzymes were the first application of genetically modified organisms in food production and were approved in 1988 by the US Food and Drug Administration.[83] In the early 1990s, recombinant chymosin was approved for use in several countries.[83][84] Cheese had typically been made using the enzyme complex rennet that had been extracted from cows' stomach lining. Scientists modified bacteria to produce chymosin, which was also able to clot milk, resulting in cheese curds.[85] The People’s Republic of China was the first country to commercialize transgenic plants, introducing a virus-resistant tobacco in 1992.[86] In 1994 Calgene attained approval to commercially release the Flavr Savr tomato, a tomato engineered to have a longer shelf life.[87] Also in 1994, the European Union approved tobacco engineered to be resistant to the herbicide bromoxynil, making it the first genetically engineered crop commercialized in Europe.[88] In 1995 Bt Potato was approved safe by the Environmental Protection Agency, after having been approved by the FDA, making it the first pesticide producing crop to be approved in the USA.[89] In 1996 a total of 35 approvals had been granted to commercially grow 8 transgenic crops and one flower crop (carnation), with 8 different traits in 6 countries plus the EU.[81]

By 2010, 29 countries had planted commercialized biotech crops and a further 31 countries had granted regulatory approval for transgenic crops to be imported.[90] In 2013 Robert Fraley (Monsanto’s executive vice president and chief technology officer), Marc Van Montagu and Mary-Dell Chilton were awarded the World Food Prize for improving the "quality, quantity or availability" of food in the world.[91]

The first genetically modified animal to be commercialised was the GloFish, a Zebra fish with a fluorescent gene added that allows it to glow in the dark under ultraviolet light.[92] The first genetically modified animal to be approved for food use was AquAdvantage salmon in 2015.[93] The salmon were transformed with a growth hormone-regulating gene from a Pacific Chinook salmon and a promoter from an ocean pout enabling it to grow year-round instead of only during spring and summer.[94]

Opposition[edit]

Opposition and support for the use of genetic engineering has existed since the technology was developed.[78] After Arpad Pusztai went public with research he was conducting in 1998 the public opposition to genetically modified food increased.[95] Opposition continued following controversial and publicly debated papers published in 1999 and 2013 that claimed negative environmental and health impacts from genetically modified crops.[96][97]

References[edit]

DNA studies suggested that the dog most likely arose from a common ancestor with the grey wolf.[1]
  1. ^ abSkoglund, Pontus; Ersmark, Erik; Palkopoulou, Eleftheria; Dalén, Love (2015-06-01). "Ancient Wolf Genome Reveals an Early Divergence of Domestic Dog Ancestors and Admixture into High-Latitude Breeds". Current Biology. 25 (11): 1515–19. doi:10.1016/j.cub.2015.04.019. PMID 26004765. 
  2. ^Jackson, DA; Symons, RH; Berg, P (1 October 1972). "Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: Circular SV40 DNA Molecules Containing Lambda Phage Genes and the Galactose Operon of Escherichia coli". PNAS. 69 (10): 2904–09. Bibcode:1972PNAS...69.2904J. doi:10.1073/pnas.69.10.2904. PMC 389671. PMID 4342968. 
  3. ^Larson, Greger; Karlsson, Elinor K.; Perri, Angela; Webster, Matthew T.; Ho, Simon Y. W.; Peters, Joris; Stahl, Peter W.; Piper, Philip J.; Lingaas, Frode (2012-06-05). "Rethinking dog domestication by integrating genetics, archeology, and biogeography". Proceedings of the National Academy of Sciences. 109 (23): 8878–83. Bibcode:2012PNAS..109.8878L. doi:10.1073/pnas.1203005109. PMC 3384140. PMID 22615366. 
  4. ^Montague, Michael J.; Li, Gang; Gandolfi, Barbara; Khan, Razib; Aken, Bronwen L.; Searle, Steven M. J.; Minx, Patrick; Hillier, LaDeana W.; Koboldt, Daniel C. (2014-12-02). "Comparative analysis of the domestic cat genome reveals genetic signatures underlying feline biology and domestication". Proceedings of the National Academy of Sciences. 111 (48): 17230–135. Bibcode:2014PNAS..11117230M. doi:10.1073/pnas.1410083111. PMC 4260561. PMID 25385592. 
  5. ^Zeder, Melinda A. (2008-08-19). "Domestication and early agriculture in the Mediterranean Basin: Origins, diffusion, and impact". Proceedings of the National Academy of Sciences. 105 (33): 11597–604. Bibcode:2008PNAS..10511597Z. doi:10.1073/pnas.0801317105. PMC 2575338. PMID 18697943. 
  6. ^the history of maize cultivation in southern Mexico dates back 9000 years.New York Times, (2010-05-25)
  7. ^Colledge, Sue; Conolly, James (2007). The Origins and Spread of Domestic Plants in Southwest Asia and Europe. p. 40. ISBN 1598749889. 
  8. ^ abcdeKingsbury, Noel (2009). Hybrid: The History and Science of Plant Breeding. University of Chicago Press. ISBN 0226437051.
  9. ^"Evolution of Wheatpublisher=Wheat, the big picture". Archived from the original on 2013-01-28. 
  10. ^Le Page, Michael (2016-03-17). "Farmers may have been accidentally making GMOs for millennia". The New Scientist. Retrieved 2016-07-11. 
  11. ^Schouten, H. J.; Jacobsen, E. (2007). "Are Mutations in Genetically Modified Plants Dangerous?". Journal of Biomedicine and Biotechnology. 2007: 1–2. doi:10.1155/2007/82612. 
  12. ^Hartl, D. L.; Orel, V. (1992). "What Did Gregor Mendel Think He Discovered?". Genetics. 131 (2): 245–25. PMC 1205000. PMID 1644269. 
  13. ^Vries, H. de (1889) Intracellular Pangenesis[1] ("pan-gene" definition on page 7 and 40 of this 1910 translation in English)
  14. ^Creative Sponge. "The Bateson Lecture". Archived from the original on 2007-10-13. 
  15. ^Roberts, R. J. (2005). "Classic Perspective: How restriction enzymes became the workhorses of molecular biology". Proceedings of the National Academy of Sciences. 102 (17): 5905–08. Bibcode:2005PNAS..102.5905R. doi:10.1073/pnas.0500923102. PMC 1087929. PMID 15840723. 
  16. ^Weiss, B.; Richardson, C. C. (1967). "Enzymatic breakage and joining of deoxyribonucleic acid, I. Repair of single-strand breaks in DNA by an enzyme system from Escherichia coli infected with T4 bacteriophage". Proceedings of the National Academy of Sciences. 57 (4): 1021–28. Bibcode:1967PNAS...57.1021W. doi:10.1073/pnas.57.4.1021. PMC 224649. PMID 5340583. 
  17. ^Lederberg, J (1952). "Cell genetics and hereditary symbiosis". Physiological Reviews. 32 (4): 403–30. doi:10.1152/physrev.1952.32.4.403. PMID 13003535. 
  18. ^Mandel, Morton; Higa, Akiko (1970). "Calcium-dependent bacteriophage DNA infection". Journal of Molecular Biology. 53 (1): 15962. doi:10.1016/0022-2836(70)90051-3. PMID 4922220. 
  19. ^Cohen, S. N.; Chang, A. C. Y.; Hsu, L. (1972). "Non chromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNA". Proceedings of the National Academy of Sciences. 69 (8): 2110–14. Bibcode:1972PNAS...69.2110C. doi:10.1073/pnas.69.8.2110. PMC 426879. PMID 4559594. 
  20. ^Wirth, Reinhard; Friesenegger, Anita; Fiedlerand, Stefan (1989). "Transformation of various species of gram-negative bacteria belonging to 11 different genera by electroporation". Molecular and General Genetics MGG. 216 (1): 175–77. doi:10.1007/BF00332248. PMID 2659971. 
  21. ^Nester, Eugene (2008). "Agrobacterium: The Natural Genetic Engineer (100 Years Later)". 
  22. ^Zambryski, P.; Joos, H.; Genetello, C.; Leemans, J.; Montagu, M. V.; Schell, J. (1983). "Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity". The EMBO Journal. 2 (12): 2143–50. PMC 555426. PMID 16453482. 
  23. ^Jackson, D. A.; Symons, R. H.; Berg, P. (1972). "Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: Circular SV40 DNA Molecules Containing Lambda Phage Genes and the Galactose Operon of Escherichia coli". Proceedings of the National Academy of Sciences. 69 (10): 2904–09. Bibcode:1972PNAS...69.2904J. doi:10.1073/pnas.69.10.2904. PMC 389671. PMID 4342968. 
  24. ^"Genome and genetics timeline – 1973". Genome news network. 
  25. ^Arnold, Paul (2009). "History of Genetics: Genetic Engineering Timeline". 
  26. ^Cohen, Stanley N.; Chang, Annie C. Y. (1973). "Recircularization and Autonomous Replication of a Sheared R-Factor DNA Segment in Escherichia coli Transformants". Proceedings of the National Academy of Sciences of the United States of America. 70 (5): 1293–97. Bibcode:1973PNAS...70.1293C. doi:10.1073/pnas.70.5.1293. JSTOR 62105. PMC 433482. PMID 4576014. 
  27. ^Jaenisch, R.; Mintz, B. (1974). "Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA". Proceedings of the National Academy of Sciences of the United States of America. 71 (4): 1250–54. Bibcode:1974PNAS...71.1250J. doi:10.1073/pnas.71.4.1250. PMC 388203. PMID 4364530.

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